DAKEWE

I. Analysis of immune cells (Traditional Flow)

1. Sample Type: Routine human or mouse samples, such as peripheral blood,PBMCs, spleen, etc.

2. Staining Panel Combination: Surface markers of immune cells, cytokines

(such as interleukins, interferons, etc.), and nuclear markers (Foxp3, Helios, Ki-67, etc.)

3. Instrument: Beckman CytoFLEX S flow cytometer

(traditional flow cytometry, up to 12 colors)

4. Service Time：Monday to Friday, 9:30–17:00

5. Pricing:

Treg cell subtype typing in mouse spleen tissue (traditional flow cytometry, 10 colors)

II. Analysis of immune cells(Spectral Flow)

1. Sample Type: Routine human or mouse samples, such as peripheral blood, PBMCs, spleen, etc.

2. Staining Panel Combination: Surface markers of immune cells, cytokines

(such as interleukins, interferons, etc.), and nuclear markers (Foxp3, Helios, Ki-67, etc.)

3. Instrument: Cytek Northern Lights 3000 spectral flow cytometer

(spectral flow cytometry, up to 25 colors)

4. Service Hours: Monday to Friday, 9:30 AM – 5:00 PM

5. Pricing:

Characterization of immune cell subsets in mouse spleen tissue (18-color flow cytometry)

III. Sorting of Specific Cell Populations

1. Sample Type: Routine human or mouse samples,

such as peripheral blood, PBMCs, spleen, etc.

2. Staining Panel Assemblies: Surface markers representing immune cells

3. Machine Model: BDFACSAria™ III SORP Flow Cytometer

4. Sorting Tubing: Up to 4 sorting channels

5. Service Hours: Monday to Friday, 9:30 AM – 5:00 PM

6. Pricing:

IV. Cell Health and Function Monitoring

1. Project Types:

   • Multi-indicator characterization of apoptosis:

   (mitochondrial membrane potential (JC-1), activated caspase level,

    everted PS groups (Annexin V, Apotracker Tetra), sub-diploid peak, etc.)

   • Cell proliferation (CFSE, BrdU) and DNA ploidy analysis

   • Cell cycle analysis (nucleic acid dye + Ki67)

   • ROS signaling molecule/calcium flow signaling molecule detection

   • Fluorescent reporter gene signal detection

   (phagocytic function, drug screening, etc.)

   • Transfection efficiency assay

2. Sample Types: Peripheral immune tissues

(peripheral blood, spleen tissue), cell lines, etc.

3. Service Hours: Monday to Friday, 9:30 AM – 5:00 PM

4. Pricing:

Proliferative status of human PBMC samples on day 5 after stimulation with 10 μg PHA (CFSE concentration 2.5 μM)